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Legionella Tests
Reviewed by Medicinsk Expert•Last updated: 2026-10-22•Lab Tests

Legionella Tests

Legionella tests are used to diagnose Legionnaires' disease, a severe form of pneumonia caused by bacteria found in contaminated water systems.

What are Legionella tests?

Legionella is a type of bacteria that can cause Legionnaires' disease, a severe pneumonia, or Pontiac fever, a milder flu-like illness. Together, these are known as legionellosis. The bacteria are found in freshwater and can spread through man-made water systems like plumbing, hot tubs, and air-conditioning systems. Infection occurs when people breathe in mist or water droplets containing the bacteria; it does not spread from person to person.

Other names: Legionnaires' disease testing, Legionellosis testing, Legionella pneumophila

Why do I need a test?

You may need testing if you have symptoms of Legionnaires' disease, which typically appear 2–14 days after exposure:

  • Cough and chest pain
  • High fever and chills
  • Headache and fatigue
  • Shortness of breath
  • Nausea, vomiting, and diarrhea
  • Confusion

Testing is critical because Legionnaires' disease can be fatal if not treated promptly with antibiotics.

What happens during the test?

Tests are usually performed on urine or sputum (mucus from the lungs). A blood test may also be used to check for prior exposure, though it cannot diagnose an active infection.

  • Urine test: A "clean catch" method is used to ensure the sample is not contaminated by skin germs.
  • Sputum test: You may be asked to cough deeply into a cup. If you cannot produce enough sputum, a salty mist may be used to help.
  • Bronchoscopy/BAL: If other methods fail, a provider may perform a bronchoscopy, where a thin, lighted tube is inserted into the airways. A bronchoalveolar lavage (BAL) may be performed during this procedure to wash the airways with saline and collect a sample directly from the lungs.

Preparation and Risks

No special preparation is needed for a urine test. For sputum or bronchoscopy, you may need to fast or increase water intake. Inform your provider of all medications, as antibiotics can affect results.

Urine and sputum collection carry no risk. Blood tests carry minimal risk of bruising. Bronchoscopy and BAL carry rare risks, including airway bleeding, infection, or a collapsed lung (pneumothorax).

What do the results mean?

A positive result likely indicates Legionnaires' disease. A negative result may suggest a different type of infection or that insufficient bacteria were present in the sample. Your provider will interpret these results alongside your medical history and other diagnostic tools, such as chest X-rays or bacterial cultures.

Frequently Asked Questions

Urine test results are usually available within 24 to 48 hours. If you are showing severe symptoms such as high fever, chest pain, or difficulty breathing, your clinician may start empiric antibiotics (often a macrolide or fluoroquinolone) right away while awaiting the test, because early treatment improves outcomes.
positive blood antibody test indicates past exposure, not an active infection. A negative urine test suggests there is no current shedding of bacteria. Nonetheless, if you have symptoms consistent with Legionnaires’ disease, your clinician may still initiate treatment based on clinical judgment.
properly collected sputum sample is generally sufficient for a definitive diagnosis. However, if the first sample is inadequate or the patient’s symptoms persist, a second sample may be requested to increase sensitivity, especially in patients who cannot produce a good specimen initially.
Bronchoscopy and bronchoalveolar lavage are considered safe, but they carry small risks such as bleeding, infection, or transient airway irritation. The procedure is usually performed under local or general anesthesia, and the benefits of obtaining a definitive diagnosis often outweigh the risks.
Source: MedlinePlus, National Library of Medicine

MedlinePlus brings together authoritative health information from the National Library of Medicine (NLM), the National Institutes of Health (NIH), and other government agencies and health-related organizations.

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